Authors: M. Taha, S.R. Singh, C. Moore, R. Agee, V.A. Dennis
Affilation: Alabama State University, United States
Pages: 194 - 197
Keywords: PLA-PEG, c.trachomis, protein, nanoparticles
Chlamydia trachomatis is a major human health pathogen because it is the most reported sexually transmitted disease. There is no effective mucosal vaccine against Chlamydia. One of the major problems of the C.trachomatis vaccine development is delivery; hence development of nanoparticles base vaccine might help to overcome this hurdle. Poly(ethylene glycol)(PEG) and poly(lactic acid) (PLA) is one of the most widely used biodegradable polymers in drug delivery systems. The objectives of this study were as follows: 1) to first successfully encapsulate and characterize a model protein namely, bovine serum albumin in combined PLA-PEG nanoparticles and 2) to similarly encapsulate and characterize an rMOMP-278 protein in this nanoparticle to serve as a vaccine delivery system for C. trachomatis. In this study BSA was efficiently encapsulated in PLA-PEG nanoparticle and characterized using Fourier Transform Infrared Spectroscopy, Zetasizer, Zeta potential, in vitro release study, and cytotoxicity assay. Our characterization studies showed that the PLA-PEG-BSA nanoparticle was small in size, stable, with minimal toxicity, and slowly and continuously release the protein. Overall, our study shows the successful characterization of our model protein (BSA) in PLA-PEG nanoparticles. An rMOMP-278 protein encapsulated in PLA-PEG is currently being evaluated as a vaccine delivery system for C. trachomatis.
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