Nanotech 2012 Vol. 3
Nanotech 2012 Vol. 3
Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)

Biosensing, Diagnostics & Imaging Chapter 1

Continuous Sensing of blood by dark-field microscopy and surface-enhanced Raman Spectroscopy

Authors: A.-I. Henry, B. Sharma, L.B. Sagle, R.P. Van Duyne

Affilation: Northwestern university, United States

Pages: 40 - 43

Keywords: chemical sensing, SERS, dark-field microscopy

Surface-enhanced Raman spectroscopy (SERS) is a rapid, label-free technique with multiplexing capabilities, making it very relevant for the sensitive real-time detection for biomedical applications , , . Demonstration of in vivo glucose detection with unprecedented sensitivity (micromolar to picomolar concentration sensitivity) in a rat model has been made recently in our group. We have developed a combined SERS-active microfluidic substrate that has been integrated with a SERS imaging system for the detection of biomarkers. The SERS substrate has been functionalized with optimized surface chemistries (mixed self-assembled monolayers) for the capture of small molecules and bacteria. Microfluidic channels on top of the SERS-active surface allow for flowing blood, which is distributed over the substrate (see Figure). SERS imaging uses a line focus parallel to the channels, which accounts for measuring the SERS signal continuously and visualize the SERS activity across the microfluidic channel with the imaging capability of this system.

ISBN: 978-1-4665-6276-9
Pages: 818
Hardcopy: $209.95