Pimpha N., Chaleawlert-umpon S., Chruewkamlow N., Kasinrerk W.
National Nanotechnology Center, TH
Keywords: anti-CD4 monoclonal antibody, CD4+ lymphocyte separation, immunomagnetic particles, poly (glycidyl methacrylate)
To develop an efficient magnetic system for separation of CD4+ lymphocytes, we design a simple, specific, and cost-effective CD4 separation system without the need for column separation technique. Novel immunomagnetic particles have been prepared for separation of CD4+ lymphocytes. The magnetic nanoparticles with a diameter of approximately 5-6 nm were first synthesized by co-precipitation from ferrous and ferric iron solutions and subsequently encapsulated with poly (glycidyl methacrylate) (PGMA) by precipitation polymerization. Monoclonal antibody specific to CD4 molecules expressed on CD4+ lymphocytes was conjugated to the surface of magnetic PGMA particles through covalent bonding between epoxide functional groups on the particle surface and primary amine groups of the antibodies. The generated immunomagnetic particles have successfully separated CD4+ lymphocytes from whole blood with over 95% purity. The results indicated that these particles can be employed for cell separation and provide a strong potential to be applied in various biomedical applications including diagnosis, and monitoring of human diseases.
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 13, 2011
Pages: 264 - 266
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4398-7138-6