Authors: S.Y. Kim, S.J. Jun, J. Min
Affilation: KyungWon University, Korea
Pages: 28 - 31
Keywords: Silica nanotube, quantum dots, gold nanoparticle, beacon, DNA sensor
A DNA senor using silica nanotubes (SNTs) was developed to detect some bacteria by barcoded SNTs. The SNTs were fabricated using anodic aluminum oxide (AAO) templates by the surface sol-gel method. To recognize each DNA sensor, quantum dots were embedded in a SNT (QD SNT) with different colors and order of colors. These effects contributed the possibility of multiplex detection. The length and morphology of completed QD SNTs were investigated by TEM and confocal microscope. The resulting QD SNTs were bonded with beacons that are designed as nucleotides with hairpin structure as a capture probe and a signal probe at the same time. At this process, the colloidal gold nanoparticle was used to bond between QD SNT and beacon by sulfur-gold bond. Furthermore, it was able to perform as a quencher to extinct a fluorescence signal of a beacon when the beacon was in normal condition. The gold-coated QD SNTs immobilized with DNA (bio-SNTs) were fabricated and investigated to confirm the operation by confocal microscope. This research can contribute the potential for the multiplex detection using gold-coated QD SNTs immobilized DNA.
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