Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy

Bio Sensors, Diagnostics & Imaging Chapter 1

Facile Fabrication of an Enzyme Microreactor Using Magnetic Microbeads

Authors: X. Liu, R.C. Lo, F.A. Gomez

Affilation: California State University, Long Beach, United States

Pages: 141 - 144

Keywords: diaphorase, enzyme, magnetic microbeads, microfluidics, microreactor

In this paper, we present a simple method to fabricate an enzyme microreactor using soft lithography and magnetic microbeads. For proof of concept, we performed an enzymatic reaction by flowing non-fluorescent resazurin through diaphorase (DI) (E.C. 1.6.99) covalently attached to magnetic microbeads packed in a PDMS microfluidic chip to convert it to fluorescent resorufin in the presence of nicotinamide adenine dinucleotide phosphate (NADH).

ISBN: 978-1-4398-3415-2
Pages: 880
Hardcopy: $189.95