Nanotech 2010 Vol. 3
Nanotech 2010 Vol. 3
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy

Bio Sensors, Diagnostics & Imaging Chapter 1

Functionalized Nanopipette: towards single cell immunoassay

Authors: P. Actis, O. Jejelowo, N. Pourmand

Affilation: UC Santa Cruz, United States

Pages: 88 - 90

Keywords: nanopipette, immunoassay, label-free, biosensor

Nanopipette technology is capable of detecting and functional analyzing bio-molecules based on difference on their size, shape and electrical charge. This unique label-free biosensor is inexpensive, easy to fabricate and versatile. Nanopipette functionalized with antibodies specific to HT-2 toxin, a food-borne pathogen, showed a limit of detection for HT-2 toxin as low as 100 fg/ml with a dynamic range of 4 logs. We are currently investigating the application of functionalized nanopipette for HPV proteins detection. A highly sensitive nanopipette probe can be precisely positioned at any sub-cellular region, thus paving the way for single cell immunoassay.

ISBN: 978-1-4398-3415-2
Pages: 880
Hardcopy: $189.95