Authors: M.K. Khan, M.S.T. Kariapper, V. Kasturirangan, B. Nair, W. Lesniak, L.P. Balogh
Affilation: Roswell Park Cancer Institute, United States
Pages: 549 - 550
Keywords: PAMAM, toxicity, composite nanodevice
Composite nanodevices, with PAMAM dendrimer and inorganic metal components, are currently being developed as an exciting nanoplatform for several types of molecularly targeted cancer therapy and imaging. Due to its unique chemical structure of dendrimers, one can attach chemical moieties of interest on the surface and/or incorporate metal atoms or therapeutic compounds inside the dendrimer molecules to form composite nanoparticles with specific use. In this study we evaluate the toxicity of three types of composite nanodevices. Toxicity was assessed using and in vitro proliferation assay in a prostate cancer cell and in primary culture human endothelial cells and the toxicity was assessed over the concentration ranges of 10nM–2uM of nanodevices and over time. First we examine Au dendrimer composite nanodevices one being developed as a radiotherapy agent, since 198Au has already been used in radiotherapy. Tin also has potential for use in x-ray based contrast imaging, and we examine the toxicity of Sn based composite nanodevices. We then also test the toxicity of a hybrid cancer therapy and imaging 5nm composite nanodevice Au-Sn-CND. Prior to any human use, these devices will need to be evaluated for cellular toxicity. This study demonstrates the range of safety of these metal composite nanodevices in both tumor and normal cell systems, and at physiologic concentrations as well as high concentration levels.
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