Authors: E.S. Gil, T.L. Lowe
Affilation: Pennsylvania State University, United States
Pages: 379 - 381
Keywords: nanoparticles, polysaccharide, degradable, blood brain barrier, insulin
The primary objective of this study is to evaluate novel cationic and degradable beta-cyclodextrin nanoparticles as drug delivery carriers for insulin across the bovine brain microvessel endothelial cell (BBMVEC) monolayer, an in vitro blood brain barrier (BBB) model. We use dynamic light scattering, Zeta potentiometer and FTIR to characterize the particle sizes, zeta potentials and degradation of the nanoparticles. We study the release kinetics of insulin from the nanoparticles. We investigate the permeability of the nanoparticles without/with Doxo and insulin across the BBMVEC monolayer. We also assess the in vivo biodistribution of the nanoparticles. Our preliminary results demonstrate that the nanoparticles can sustain release of insulin, and are not only twice as permeable as 4 kD dextran control, but also enhance insulin permeability across the BBMVEC monolayer.