Nanotech 2007 Vol. 4
Nanotech 2007 Vol. 4
Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 4

Nanofabrication Chapter 3

The Si-tag for Immobilizing Proteins on a Silicon Device

Authors: A. Kuroda

Affilation: Hiroshima University, Japan

Pages: 571 - 574

Keywords: protein, immobilization, biosensors, supramolecular assemblies

Targeting functional proteins to specific sites on a silicon device is essential for the development of new biosensors and supramolecular assemblies. Recently, we found ribosomal protein L2 as a silicon-binding protein, which can be used to immobilize fused proteins on a silicon device. Indeed, a fusion of L2 and green fluorescence protein (GFP) adsorbed to the silicon/silica particles with a Kd of 0.7 nM at pH 7.5. This fusion protein was retained on the silicon device even after washing for 24 h with a buffer. A fusion of L2 and firefly luciferase also functionally adsorbed on the silicon device. This L2, which we call the Si-tag, can be used for one-step targeting of functional proteins on a silicon device without chemical modification of the surface.

ISBN: 1-4200-6376-6
Pages: 768
Hardcopy: $139.95