Nanotech 2005 Vol. 2
Nanotech 2005 Vol. 2
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 2

NEMS and MEMS Fabrication Chapter 7

Microfabricated Silicon Apertures for Ion Channel Measurement

Authors: S.J. Wilk, M. Goryll, L. Petrossian, G.M. Laws, S.M. Goodnick, T.J. Thornton, M. Saraniti, J.M. Tang and R.S. Eisenberg

Affilation: Arizona State University, United States

Pages: 504 - 507

Keywords: ion channels, microfabrication, membranes

Ion channels are proteins that act as pores in cell membranes and allow only specific ions to pass through the cell wall. Here, we present a method for microfabrication of apertures in a silicon substrate using well-known cleanroom technologies. Apertures were etched through a silicon wafer using a plasma-etcher and then rendered hydrophobic with chemical vapor deposition of a Teflon surface layer. Characteristic measurements showing physiological single channel gating behavior of OmpF porin and alpha-hemolysin channel proteins have been made.

ISBN: 0-9767985-1-4
Pages: 808
Hardcopy: $109.95