Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1

Computational Biology Chapter 9

A Multi-scale, Integrative Model of Cellular Electrophysiological Response Incorporating Intra/Extra-cellular Transport & Dynamics

Authors: J.J. Feng, S. Krishnamoorthy and S. Sundaram

Affilation: CFD Research Corporation, United States

Pages: 548 - 551

Keywords: multiscale model, ion channel, deconvolution of AP, simulation

Spurred by the growing confluence of nano and bio technologies, several novel micro/nano platforms (see for example, whole cell patch clamp devices [1]) are emerging for the high-throughput and/or high-fidelity study of the electrophysiological response of a single cell (or a few cells) to external excitation (potential, ion-induced depolarization or hormonal changes etc.). However, a significant challenge in interpreting the data from the instruments is the de-convolution of transport-related effects from pure electrophysiological phenomena, such as the gated responses. In response to this challenge, we have developed a novel multi-scale approach (Figure 1) in which, high-fidelity spatio-temporal models describing intra and extra cellular fluid motion and ion transport (e.g. representing calcium store dynamics) are coupled with an equivalent electric circuit network model describing ion-channel activation. In addition to a clear separation of the transport from the electrophysiology, and thereby more rigorous data analysis, the proposed model will also enable the knowledge-based design and development of novel patch-clamp experimental platforms.

ISBN: 0-9767985-0-6
Pages: 844
Hardcopy: $109.95