Authors: C-J Shih, W-W Ke, C-H Lieu
Affilation: ITRI, Taiwan
Pages: 474 - 476
Keywords: drug screen, acoustic wave sensor, flexural plate wave, SARS
The Severe Acute Respiratory Syndrome (SARS) is induced to the patients pervasively pneumonia and respired exhaustion. In previously study (2), the nucleic acid 318 and 510 of S protein was the crucial fragments to bind angiotensin-converting enzyme 2 (ACE2), which was very recently identified as a functional receptor for the SARS virus. For developing anti-SARS drug in traditional way, it’s usually found out the chemical compound with anti-SARS capability to test tissue or animal with infective SARS virus. In this study, we develop a new method to rapidly and simply screen drugs, which can interfere with the interaction between ACE2 and S protein. Electrostatic attraction and molecular recognition, S protein and hACE2, were employed to the functionalized Flexural Plate Wave (FPW) biosensor via Ni2+ chemical reactions. In our research, FPW sensor was used to distinguish the biomaterial recognizable property between adsorption of biomaterial and drugs precursor. We try to detect an anti-SARS drug by the measurement of frequency shift of FPW sensor due to the variation of weight. In our system, an anti-SARS drug transmit into sensor system with immobilization by S-hACE2 hybrid protein in Fig.1, it would destroy interaction between hACE2 and S protein by blocking S protein activity site. Furthermore, the results obtained by FPW measurements would be compared with the traditional methods such as gene engineering, PCR and western blot. Transfering gene and cloning S protein or hACE2 into Sf-9 insect cell and confirming protein activity were shown as in Fig.2, Fig.3 and Fig.4.
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