Nanotech 2005 Vol. 1
Nanotech 2005 Vol. 1
Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1

Bio Micro Sensors Chapter 8

Effect of Length of Biotin Modified n-Alkanethiolates on the Formation of a Self-Assembled Streptavidin-Biotin Monolayer on a Gold Surface

Authors: D.-J. Kim, E.C. Cho and K.-K. Koo

Affilation: Sogang University, Korea

Pages: 438 - 441

Keywords: SAM, streptavidin, biotin

The molecular recognition between streptavidin and biotin makes the streptavidin-biotin system very useful in a wide range of biotechnological applications. Currently, biotin-modified n-alkanethiolates have been extensively studied to create a wide variety of self-assembled monolayers (SAMs) on gold surfaces. For the formation of optimum monolayer films, an appropriate biotin-terminated molecule should be selected to minimize nonspecific interactions between streptavidin and the modified surface and to maximize specific binding between them. In the present work, various monolayer films with adsorption of biotin-terminated long-chain n-alkanethiolates and of mixtures with 11-mercaptoundecanol (MUOH) from solution onto the gold surfaces were prepared and characterized by optical ellipsometry, contact angle goniometry, X-ray photoelectron spectroscopy (XPS), and scanning tunneling microscopy (STM).

ISBN: 0-9767985-0-6
Pages: 844
Hardcopy: $109.95