Authors: R.M. Jendrejack, J.J. de Pablo and M.D. Graham
Affilation: University of Wisconsin-Madison, United States
Pages: 88 - 91
Keywords: DNA, microfluidic, hydrodynamic interactions, Brownian dynamics, polymer solutions
Simple arguments predict that the dynamics of a dissolved macromolecule confined to a channel comparable to its equilibrium coil size (~1 um for viral DNA) are quite different from those in free solution, because of the no-slip boundary condition on the fluid motion. Nevertheless, detalied, predictive computations have not been previously performed. We have incorporated a Brownian dynamics model of DNA into a fully self-consistent computational scheme that simultaneoulsy resolves the macromolecular and fluid (i.e. solvent) dynamics of DNA in a microfluidic channel. The key novel feature of this scheme is the numerical computation of the Green's function for the flow problem, enabling a stochastic solution method that incorporates detailed hydrodynamics and respects the fluctuation-dissipation theorem. With this methodology we study a number of important confinement effects, focusing here on the retardation of relaxation of a chain in small channel.
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