Authors: S. Krishnamoorthy, V.B. Makhijani, M. Lei, M.G. Giridharan and T. Tisone
Affilation: CFD Research Corporation, United States
Pages: 590 - 593
Keywords: rapid diagnostics, lateral-flow, simulation, membrane, quantitation
Membrane-based test formats provide simple, accurate and cost effective diagnoses of acute and chronic conditions in general population health. The sensitivity of the immunoassay is determined by the complex biochemical binding kinetics associated with the analyte and receptor molecules in the system. In most flow-through diagnostic systems, the analyte-receptor binding is influenced by the transport of analyte molecules to the receptor surface, which in turn is governed by the flow of sample in the system. Thus, understanding the flow of sample and transport of analyte molecules in a membrane is a crucial step in designing an optimum assay. In the present work, this is accomplished with the help of computational models (CFD-ACE+ from CFD Research Corporation). Line sensitivity analysis and formation of immobile reagent are discussed in the context of a lateral-flow test strip.
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