NSTI Nanotech 2009

Covalent biofunctionalization of noncovalently immobilized fluorous thinfilms using click chemistry

C.M. Santos, A. Kumar, W. Zhang, C. Cai
University of Houston, US

Keywords: biofunctionalization, click chemistry, fluorous films


A practical method for surface immobilization of biomolecules on fluorous substrates via click chemistry have been developed. The thin films with ethynyl handles are readily prepared on commercial fluorous glass slides, and such non-covalently immobilized thin films are surprisingly stable, allowing covalent biofunctionalization in good yields (70-80%) via CuAAC reactions in aqueous or suitable organic solvents. This method is complimentary to the direct deposition of fluorous-tagged compounds. Furthermore, the same approach can be used for other “click” reactions on fluorous surfaces.
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