NSTI Nanotech 2009

Development of Novel Landscape Phage Libraries and Their Use for Selection of Phage Probes for Staphylococcus aureus

C. Xu, S.B. Price, S. Kitchens, V.A. Petrenko
Auburn University, US

Keywords: phage display, landscape phage library, staphylococcus aureus


Filamentous phage fd (and its close relatives M13 and f1) represent a unique natural nanomaterial, whose structure and function are encrypted in its single-stranded DNA genome and can be tailored by molecular genetic means. Libraries of landscape phage - multibillion clone collections of phages with highly diverse surface landscapes - serve as a rich source of novel ligands for various receptors, which can be isolated by affinity selection (biopanning). However, due to the limitation of the existing libraries in size, diversity and repertoire, finding of an optimal ligand might be a problem. We hypothesize that by changing the conformation and format of the inserts in the major coat protein, we could construct new libraries with increased size, diversity and distinct repertoire. In this study, two new libraries and previously designed libraries f8/8 and f8/9 will be analyzed statistically and screened against Staphylococcus aureus bacteria to characterize their complexity (size), diversity and repertoire in relation to different formats and presentation of the inserted peptides.
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