2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

High Resolution Labeling of Proteins Implicated in Central Nervous System Injury and Disease using Quantum Dot Nanocrystals

S. Pathak, G. Silva
University of California, San Diego, US

quantum dot nanocrystals, gliosis, central nervous system regeneration

High-resolution imaging of proteins is crucial for the visualization of changes in these protein expressions in disease and injury. In the central nervous system (CNS), a variety of proteins have beem implicated as being barriers to regeneration. However, this process is still not well understood. In the retina, for example, glial fibrilliary acetic protein (GFAP) upregulation is a known result due to injury or disease. The mechanism for GFAP upgreguation and propagation however remains uknown. Correlating GFAP upregulation with other proteins of interest is important on a variety of levels. It will provide targets for pharmaceutical drugs as well as contribute to the basic understanding of this disease process. In this study, we used quantum dots (Qdots) to achieve extremely high resolution labeling of GFAP and integrins, associated with gliosis in the rat neural retina. This represents the first time Qdot labeling has been achieved in the neural retina. Due to the extreme detail that results with Qdot labeling, the glial scarring was successfully modeled and quantified. Disease models such as choroidal neovascularization (CNV) were also correlated to gliosis in the laser-induced rat CNV model.

Nanotech 2008 Conference Program Abstract