2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Hydrogenase in the Cyanobacterium Synechococcus sp. PCC 7002: Transcriptional Regulation and Overexpression

Y. Xu, D.A. Bryant
Penn State University, US

transcriptional regulation, hydrogenase, lexA, overexpression

The marine, unicellular cyanobacterium Synechococcus sp. PCC 7002 synthesizes a bidirectional [NiFe] hydrogenase (H2ase) encoded by five hox genes (hoxE, hoxF, hoxH, hoxU, hoxY). Maturation of this H2ase requires seven hyp/hox gene products (HypA, HypB, HypC, HypD, HypE, HypF and HoxW). Through RT-PCR and Q-RT-PCR, we have found several conditions or mutants that led to increased mRNA level of hox and hyp genes. LexA does not appear to regulate transcription of the hox-hyp operon. A lexA null mutant appears to have a defect in carbon metabolism and grows slower than WT at air-level of CO2. Mutation of lactate dehydrogenase gene tremendously changed the composition of fermentative metabolites and led to two-fold increase of H2 production. We have profiled whole cell hydrogenase activity by measuring H2 production from wild-type cells under indicated conditions.

Nanotech 2008 Conference Program Abstract