2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Lateral-Driven Continuous Dielectrophoretic Separation Technology for Blood Cells suspended in a Highly Conductive Medium

J. Seo, J. Jung, S-I. Han, K-H Han, Y. Jung, A. Bruno Frazier
Inje University, KR

blood cell, cell, dielectrophoresis, lateral-driven, microfluidic

This paper presents lateral-driven continuous dielectrophoretic (DEP) microseparator for separating red and white blood cells suspended in a highly conductive dilute whole blood. The DEP microseparators enable the separation of blood cells with continuous operation based on the lateral DEP force without the use of additives such as tagging or probing materials. Experimental results show that divergent type DEP microseparator can continuously separate out 87.0% of the red blood cells (RBCs) and 92.1% of the white blood cells (WBCs) from diluted whole blood, while convergent type can separate 93.6% of the RBCs and 76.9% of the WBCs, within 5 minutes simply by using a 2-MHz 3-Vp-p AC voltage to create a gradient electric field in suspension medium with a high conductivity of 17 mS/cm.

Nanotech 2008 Conference Program Abstract