2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Unspecific uptake of M13-type bacteriophage into human endothelial cell lines.

O.A. Mandrup, P. Kristensen
University of Aarhus, DK

Phage, uptake, unspecific, endothelial cells

Targeted phage particles have been tested, as vehicles for gene delivery in mouse models1. It has previously been shown that large numbers of phage particles could be obtained from tissues of a patient infused with a phage library2, we therefore decided to look at uptake of phage particles into endothelial cells. We looked at the unspecific uptake of KM13 (a M13-type helperphage) into human endothelial cells. Cells were incubated with phages at 37 C, then washed extensively in order to remove phages sticking to the surface of the cells. We found a considerable uptake of phages into the endothelial cells. To rule out that the phages could come from the outside, a similar incubation and washing was made with cells at 4 C to prevent uptake and the results showed that no phage could be retrieved. Human fibroblasts were similarly tested for unspecific uptake at 37 C, and no phage could be retrieved. Unspecific uptake of phage particles into human endothelial cells lining the blood vessels can pose a problem in the attempt to use phage particles as vehicles in delivery and a better understanding of the underlying mechanisms is needed to circumvent potential problems with phage-derived vehicles for gene and drug delivery. 1 A. Hajitou, M. Trepel, C. E. Lilley et al., Cell 125 (2), 385 (2006). 2 W. Arap, M. G. Kolonin, M. Trepel et al., Nature medicine 8 (2), 121 (2002).

Nanotech 2008 Conference Program Abstract