2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Biocompatible, Superparamagnetic, Flame Synthesized Iron Oxide Nanoparticles: Cellular Uptake and Toxicity Studies

K. Buyukhatipoglu, A. Morss Clyne, T.A. Miller
Drexel University, US

nanoparticles, toxicity, cellular uptake, flame synthesis

Superparamagnetic iron oxide nanoparticles, including hematite (Fe2O3) and magnetite (Fe3O4), are widely used in applications such as targeted drug delivery, magnetic resonance imaging, tissue engineering, gene therapy, hyperthermic malignant cell treatment, and cell membrane manipulation. In the current work, superparamagnetic iron oxide nanoparticles were produced using a flame synthesis method, which provides significant advantages over other material synthesis processes. This study focuses on the interaction of flame synthesized iron oxide nanoparticles with porcine aortic endothelial cells and compares the results to those obtained using commercially available iron oxide nanoparticles. The materials characteristics of the flame synthesized iron oxide nanoparticles, including morphology, elemental composition, particle size, and magnetic properties, were analyzed by electron microscopy (TEM, ESEM, EDS), Raman Spectroscopy, and Alternating Gradient Magnetometry (AGM). Nanoparticle biocompatibility was assessed by incubating flame synthesized and commercially available iron oxide nanoparticles with endothelial cells for 24 hours. Both trypan blue and Live/Dead cell assays showed no significant toxicity difference between flame synthesized and commercially available nanoparticles. Cells exposed to both types of nanoparticles maintained membrane integrity, as indicated by minimal lactase dehydrogenase release. Endothelial cells imaged by ESEM and confirmed by EDS demonstrated that uncoated flame synthesized nanoparticles are ingested into cells in a similar manner to commercially available nanoparticles.

Nanotech 2008 Conference Program Abstract