2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

Characterization of magnetotactic bacteria from marine environment of Colombia

S.P. Grisales, V.K. Morillo, M.A. Márquez, A. Morales, U. Lins
Universidad Nacional de Colombia, CO

magnetotactic bacteria, magnetosomes, nanobiomagnetite

Samples of sediment and water were collected from Cispata bay (9°20’–9°23’N, 75°40’–75°50’W) (Colombian Caribean), in order to isolate magnetotactic bacteria (MTBs). The samples were incubated in the laboratory under low-light conditions and room temperature, for several weeks. After this, MTBs were isolated using a magnetic field. Transmission electron microscopy of cells was done operating at 80 kV. Finally, microanalysis of magnetosomes and globules was done. The results shows magnetotactic coccoids, ranging in diameter between 0,5 to 1 m. Every cell shows one or two chains of magnetosomes, constituted of elongated prismatic crystals of magnetite (identified by EDS spectroscopy), about 125 and 130 nm in sizes. EDS analysis of the globules shows mainly sulphur.

Nanotech 2008 Conference Program Abstract