2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Nanoparticles for imaging of transmembrane potential

R.A. Khatchadourian, S.J. Clarke, C. Hollmann, J.L. Nadeau
McGill University, CA

nanoparticles, quantum dots, imaging, membrane, potential

The ability to visually observe membrane potential would be extremely advantageous for monitoring cell health and activity in any cultured neuron system. On sufficiently long time scales, it would be possible to quantify cell health in response to depolarization and/or hyperpolarization. Moreover, if the observer has sufficient time resolution, action potentials may be resolved, thus allowing for optical recordings from ensembles of neurons without growing cultures on microelectrode arrays. Creating such probes remains extremely challenging. Here we present preliminary results for two genetically-encoded systems: one based upon Forster Resonance Energy Transfer (FRET) between nanoparticles and an engineered channel, and the second based upon fluorescence complementation (FC). The results suggest that regular monitoring of neuronal potential is possible with these probes, at least on relatively long periods on the scale of hundreds of milliseconds.

Nanotech 2008 Conference Program Abstract