2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Development and Utilization of Polymer-Embedded Gold Nanoparticles for Direct Fabrication of Amperometric NanoBiosensors for Nitrate and Phosphate

S.B. Adeloju, Q. Ameer, M. Sohail
Monash University, AU

Gold nanoparticles, nanobiosensors, enzymes, nitrate, phosphate

There has been a considerable interest in recent years in the use of nanoparticles, such as carbon nanotubes and metallic nanoparticles, for fabrication of chemical and biochemical sensors. In particular, the unique electronic, optical and catalytic properties offered by metallic nanoparticles of copper, gold and silver has attracted most interest. Of these, gold nanoparticles have some special features that makes it more suited for the fabrication of nanobiosensors, but requires deposition of the nanoparticle to achieve effective sensing. Many of the reported approaches for the deposition of gold nanoparticles are not suited for the direct fabrication of nanobiosensors because of the long multi-step procedure involved in the deposition. Ideally, a suitable approach for direct fabrication of nanobiosensors should involve a single step approach which does not only enable deposition of the gold nanoparticles, but also co-immobilization of the required enzyme(s), co-factor and, where necessary, the mediator. In this paper, we will describe an electrochemical strategy for embedding gold nanoparticles into conducting polymers by galvanostatic polymerization. Evidence of the incorporation of the nanoparticles into the conducting polymers will be demonstrated by SEM and electrochemical measurements. Furthermore, we will demonstrate the ability to co-immobilize two different enzyme systems as well as co-factors and mediators with the embedded nanoparticles for the amperometric biosensing of nitrate and phosphate. The successful use of the resulting amperometric nanobiosensors for the detection of nitrate and phosphate in natural waters will also be reported.

Nanotech 2008 Conference Program Abstract