2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Application of a Raman Microprobe to Monitoring Diffusion and Reactions in the Microchannels of a Lab-on-a-Chip

F. Adar, E. Lee and A. Whitley
Horiba Jobin Yvon, US

lab-on-a-chip, Raman microscope, diffusion, chemical reactions

The Raman effect is a light scattering phenomenon which provides detailed molecular information with 1 m (micrometer) spatial resolution when done on a Raman microscope. Because it is an optical technique, the spectra can be acquired in many different types of vehicles – the only requirement is that the sample of interest is optically accessible, that is, that the container is transparent. In addition, because the Raman signal of water is rather weak, water can be an ideal solvent in which to monitor flow and reactions. Since high quality spectra can be recorded as rapidly as tenths of a second, it is an ideal detector for Lab-on-a-Chip (LOC) applications; signals can be recorded as a function of time at a given spot, or can be scanned along a channel. In addition, the various species can be mapped so that Raman images can be used to measure the flow characteristics and indicate the locations of the reactants and products. Examples illustrating these effects will be shown.

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