2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Multifunctional Bio-Nano Patterns Derived from Colloidal Self-Assembly as Model Surfaces to Study Antigen-Antibody Interactions

S. Krishnamoorthy, J.P. Wright, O. Worsfold, T. Fujii and M. Himmelhaus
Fujirebio, Inc., JP

antibodies, bio-interfaces, biosensing, colloidal lithography, nanopatterning

Nanosphere lithography (NSL) has become a popular tool as a patterning technique recently because the method is cheap and involves extremely simple procedures compared with other nanopatterning techniques. NSL can also be applied to a wide range of organic and inorganic materials. If combined with selective self-assembly of organic monolayers, NSL provides a feasible tool for the bio-functionalization of substrates as future biosensors or other bio-mimetic devices. This paper presents a simple and effective means of preparing nanopatterns made of organic self-assembled monolayers on large areas by means of NSL and exploring the capability of these surfaces for oriented immobilization of antibodies. The activity of antibodies on the chemically patterned surface is compared with their un-structured counterparts, and the influence of surface topography and chemistry on the activity of the surfaces towards ELISA assays is presented. The antibody immobilization, orientation and the surface activity were followed using UV/visible, FT-IR, and surface plasmon resonance spectroscopies.

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Nanotech 2007 Conference Program Abstract


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