2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Nanoscale Coupling of a Biosensor Screening with Mass Spectrometry Confirmation

W. Haasnoot, G.R. Marchesini, O. Jansson, J. Buijs and M.W.F. Nielen
RIKILT-Institute of Food Safety, Wageningen UR, NL

surface plasmon resonance, mass spectrometry, coupling, nanoscale

The development of surface plasmon resonance (SPR)-based biosensor inhibition assays for the screening of therapeutic drugs (quinolones) in animal products is one of the topics under development within the EU-BIOCOP project (www.biocop.org). One of the results was a multi-quinolones biosensor immunoassay (BIA) which could detect al least five different fluoroquinolones This assay was combined with a specific BIA for flumequine in a dual BIA format. Another challenge within this project is to evaluate strategies for coupling the biosensor screening methods with mass spectrometry for the confirmation of the identity and/or the identification of unknowns having activity in the biosensor assays. A parallel coupling of the dual BIA with liquid chromatography electrospray time-of-flight mass spectrometry was described earlier (1). The second challenging approach is the serial coupling of the BIA screening with mass spectrometric confirmation and the application of a system using a nanoscale immunoaffinity recovery biosensor chip in combination with nano LC/TOF MS is demonstrated. 1. Marchesini, G..R., Haasnoot, W., Delahaut, P., Gerçek H., Nielen, M.W.F. Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry. Anal. Chim. Acta (In press).

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