2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Homogeneous Bioluminescence Resonant Energy Transfer On High Density Array Imaging Chip

B. Filanoski, S. Rastogi, E. Cameron, N. Mishra and W. Maki
University of Idaho, US

bioluminescence, BRET, Imaging chip, microarray, bio-detection

Fluorescent microarray technologies are widely used in molecular biology for studying gene expression, immune detection and drug discovery. Fluorescent assays are very sensitive but present problems with many bio-agents having inherent fluorescence which interfere with assays. In addition, auto-fluorescence and photo-bleaching are common problems in fluorescent detection. Moreover, fluorescence detection requires an expensive external light source coupled to expensive detection equipment. In the field of clinical diagnostics a sensitive and rapid homogeneous immunoassay is needed for harmful bio-agent detection. This paper proposes a rapid and potentially sensitive micro-chip detector based on Bioluminescence Resonance Energy Transfer (BRET). Bio-luminescent assays are exquisitely sensitive and not limited by overlap between fluorescent properties of assay components, and achieve a high signal to noise ratio. To achieve high spatial resolution, a new homogeneous bioluminescence resonance energy transfer method for imaging chip based high density array technology is presented. The distance between donor and acceptor as a way to conjugate fluorescent dyes directly to alkaline phosphatase is minimized in the new work. As a result, the efficiency of resonant energy transfer and achieved spatial resolution is increased. This method allows direct measurement of a fluorescent signal on an imaging chip without conventional optical instruments.

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Nanotech 2007 Conference Program Abstract


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