2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Imaging of Collagen Fibrils of Cartilage at Nanoscale Resolution

H.A. Alhadlaq
King Saud University, SA

imaging, collagen, nanoscale resolution

New developments, in advanced micro studies and nanotechnology, led to new discoveries when scaling down from micro to nanometer resolution. With the emerge of new exciting fields such as nanotechnology, the aim was to take advantage of the ability of quantitative Polarized Light Microscopy (PLM) and Piezoresponse Force Microscopy (PFM) to visualize biological materials at high resolution. Quantitative PLM studies of biological materials have been shown to offer useful analysis of structural information at micro and 100 nm scale resolution. It has the ability to construct quantitative 2D images at 340-136 nm/pixel resolution. One-dimensional profiles of cartilage tissue, extracted from the 2D images, were vital to study changes in the ultrastructure of cartilage tissue at a very high spatial resolution. The digital PLM method is an effective tool in the study of ultrastructural of cartilage and other biomaterials at a nanoscale resolution. Several new imaging techniques, such as the PFM, might offer better understanding at the ultrastructural level at a very high plain resolution (10s nm). High resolution images of collagen fibrils of rabbit cartilage will be presented. The employment of multiple advanced imaging techniques at micro and nanoscale resolutions are essential in understanding the structure and behavior of biological materials.

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Nanotech 2007 Conference Program Abstract


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