Nanotechnology Conference and Trade Show - Nanotech 2006
> Program > Technical Conferences > Business & Development > Nano Impact Workshop > Nanotech Job Fair > Expo
Index of Authors
Index of Keywords
Confirmed Speakers
Conferences & Symposia

Conference Proceedings

Conference Technical Proceedings

Increasing the Thermal Stability of Self-Assembling DNA Nanostructures by Incorporation of isoG/isoC Base Pairs

K. Ho, H. Li, E.B. Roesch, C.B. Sherrill, J.R. Prudent and T.H. LaBean
Duke University, US

DNA nanostructure, self-assembly, isoG/isoC

Self-assembling DNA nanostructures are currently being developed for a variety of applications in nanofabrication and molecular computing. For many uses, these nanostructures would benefit from increased thermal stability. However, increasing the lengths of sticky-ends that encode interactions between building blocks (DNA tiles) could have an adverse effect on the observed rates of assembly errors and therefore be undesirable. An alternative approach described here, makes use of the recently developed, non-natural basepair isoG/isoC which provides specific complementary pairing with three hydrogen bonds and interaction strength comparable to G/C pairing. In this study, two types of DNA cross tile lattice were compared 1) original design and 2) isoG/isoC design in which all A/T basepairs in the tile sticky-ends were substituted with isoG/isoC basepairs. We observed an increase in lattice melting temperature of approximately 11°C from Tm = 42±1°C to Tm = 53±1°C, and at the same time there was no change in the tile melting temperature (Tm = 62 ±1°C). We have successfully demonstrated a marked increase in the thermal stability of a complex DNA nanostructure via the incorporation of isoG/isoC basepairs. Future studies will include incorporation of isoG/isoC within the tile building blocks in order to increase tile stability.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2006 Conference Program Abstract

Nanotechnology Conference | Terms of use | Privacy policy | Contact | NSTI Home
Program | Technical Conferences | Business & Development | Nano Impact Workshop | Nanotech Job Fair | Expo |
Nanotech 2006 Home | Press Room | Venue | Subscribe | Site Map
Names, and logos of other organizations are the property of those organizations and not of NSTI.
This event is not open to the general public and NSTI reserves the right to refuse admission and participation to any individual.