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Ultramicro Protein Arrays for Cytokine Detection

K. Radke, K. Ryckman, S. Nettikadan, M. Lynch, C. Mosher, J. Xu and E. Henderson
BioForce Nanosciences Inc., US

ultramicro arrays, cytokine, femtogram

Several solid phase microarray based methods for the detection of the genomic materials have been developed based on amplifying the target molecules. Since proteins cannot be amplified, a similar approach to protein detection faces some unique technical hurdles. The spot size limitation of microarray methods is a primary contributor to these difficulties. We have developed instrumentation (NanoArrayer), surface patterning tools (SPTs) and methodology to construct functional ultramicro biomolecular arrays We will describe the application of ultramicro antibody arrays for the capture and detection of mouse cytokines from small (1 ul) volumes. Specificity and sensitivity data from different cytokine captures will be presented. The cytokine capture was specific and had sensitivity in the order of femtograms. This level of sensitivity from low volumes of samples should be sufficient to analyze cell lysates from a few cells, laser capture microdissection (LCM) samples and biopsy samples.

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