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Nano Science and Technology Institute 2005 NSTI Nanotechnology Conference & Trade Show
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Guided Neurite Growth on Patterned Carbon Nanotubes

X. Zhang, S. Prasad, S. Niyogi, M. Ozkan and C.S. Ozkan
University of California, Riverside, US

carbon nanotube, neuron, scaffold

Nanomaterials substrates are suitable for functioning as cell scaffolds. Promotion of axonal growth and support for long distance regeneration are the two requirements in the various experimental strategies for spinal cord repair. Organic systems’ performance is based on molecular behaviors at nanoscale. The significant advantage of nanomaterials is in their capability of acheiving control at molecular and supramolecular levels. This makes them highly attractive in bioapplication. This is the first time we demonstrate using patterned vertically growth CNT to guide axon extension. A combination of microlithography and chemical vapor deposition is used to engineer patterned vertical multiwalled carbon nanotube substrates. These substrates function as scaffolds and are used to demonstrate the formation of directed neuronal networks. Multiple substrate geometries and nanotube heights were fabricated to determine the most suitable combination for understand the cell morphological changes. Changes in the interaction between the cell membrane and the nanotube substrate are visually characterized. Cell viability was determined using calcium stains.

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