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Design and Applications of a Novel Type of Hairpin Probes: Addressable Bipartite Molecular Hook (ABMH).

J.B.P. Landré, A. Ruryk, T. Schlicksbier, S. Russwurm and H.-P. Deigner
University of East Anglia, UK

molecular beacons, microarray, immobilization

ABMHs but not TMBs display the characteristic melt curves obtained with MBs. Moreover, ABMHs specificity in solution (Figure 2) is similar to those of MBs (i.e. differentiation between a perfect match and a 1 mismatch target). The spotting protocol involves only oligonucleotides and there is no need to preserve a secondary structure as for MBs and TMBs. ABMH structures allow the powerful combination of target hybridisation in solution and subsequent specific localisation on an array. The loss in signal/noise ratio is considerably lower than what has been reported to date. Fang, et al., 1999 reported a (S/N) loss using classic modified MB is nearly 10 fold using ABMH the loss in signal is 1.6 fold. ABMHs could also differentiate between a perfect match and a 1 mismatch target on slide. Conclusions: ABMHs utilize a novel structural approach (Figure 1C) which solves most of the problems associated with the use of MBs and TMBs technologies. ABMHs allow specific, leakage free localisation of the structures with greatly improved immobilisation, structure formation, hybridization and reduced loss in signal. They thus appear ideally suited for the development of diagnostics, lab-on-chips, etc.

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