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Apoptosis of the Human Neutrophils under a Lipopolisaccharide (LPS) of Proteus Mirabilis Condition Observed in Real Time by Atomic Force Microscopy (AFM).

S.N. Pleskova, Yu. Guschina and M.B. Zvonkova
Medical State Academy Nizhniy Novgorod, RU

apoptosis, AFM

The goal of present work was to receive the images of apoptosis process in dynamics after addition of Proteus mirabilis 210 LPS and to measure the mechanical properties change of neutrophils’ membranes. We observed the significantly decrease of respiratory burst intensification on 15 min. after addition 0, 1 ml LPS of Proteus mirabilis 210 by luminol-dependent chemiluminescence. The same result was received by AFM. Change of cells morphological structure was exposed on 10 min after incubation with LPS. The volume of cells increased in 1, 8 – 2, 1 times on 15 min after addition LPS. We studied formation and separation of the apoptosis bodies after 50 min observation. Cells changed the morphological parameters (volume and height) during 3 h. Young’s module was used for estimated the mechanical properties of neutrophils’ membranes. Young’s module was 2, 2 kPa before addition of LPS and 3, 5 kPa after it. So, in present paper it has established the increase of cells membranes rigid and the increase of volume and height of neutrophils after addition of LPS P. mirabilis 210.

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