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Structural and Chemical Micropatterns Based on Zeolite Materials

K.F. Lam, S.M. Lai, W.Q. Sun, L.W. Wong and K.L. Yeung
The Hong Kong University of Science and Technology, CN

zeolite, micropattern

Compositional micropatterns (i.e., 10-100 microns) were created from structurally similar hydrophobic Sil-1 and hydrophilic ZSM-5 zeolites, and tested for the immobilization of fluorescent dyes and biomolecules. The effects of pattern size, height and geometry were investigated in detail. Being inorganic, the zeolite micropattern does not deteriorate with time and can be readily reused by simply burning away the deposited chemicals and biomolecules. Fluorescein isothiocyanate (FITC) dissolved in dimethylsulfoxide (DMSO) were preferentially deposited onto hydrophobic Sil-1 patterns regardless of the pattern geometry. Similarly, FITC-conjugated Bovine Serum Album (BSA) molecules also deposited on Sil-1 creating a regular array pattern (Fig. 1). It was clear from the fluorescent picture in Fig. 1b that the BSA molecules were uniformly deposited. This suggested that compositional micropatterns of zeolites could be used to create high resolution array of biomolecules on flat substrate.

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