Nano Science and Technology InstituteNano Science and Technology Institute
Nano Science and Technology Institute 2005 NSTI Nanotechnology Conference & Trade Show
Nanotech 2005
Bio Nano 2005
Business & Investment
Nano Impact Workshop
Index of Authors
Index of Keywords
Keynote Presentations
Confirmed Speakers
Participating Companies
Industry Focus Sessions
Nanotech Expo
Special Symposia
Venue 2005
Press Room
Site Map
Nanotech 2005 At A Glance
Nanotech Proceedings
Nanotechnology Proceedings
Global Partner
nano tech
Supporting Organizations
Nanotech 2005 Supporting Organization
Media Sponsors
Nanotech 2005 Medias Sponsors
Event Contact
696 San Ramon Valley Blvd., Ste. 423
Danville, CA 94526
Ph: (925) 353-5004
Fx: (925) 886-8461

Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA

H.X. Li and L.J. Rothberg
University of Rochester, US

PCR, DNA, gold nanoparticle, colorimetric detection, Au nanoparticles

Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all assays for DNA are based on the polymerase chain reaction (PCR), a rapid, inexpensive and simple means of amplifying specific sequence segments from as little as a single copy of DNA to easily detected quantities5, 6. Post processing of PCR amplified samples can, however, be expensive and time-consuming7. Here we demonstarte a label free colorimetric assay for SNPs in PCR amplified genomic DNA within ten minutes without need of time-consuming labeling, surface functionalization processes and additional detection instrumentation8. This assay is based on our new observation that single stranded DNA (ss-DNA) adsorbs on gold nanoparticles (Au-np) with a rate that depends on sequence length and temperature, and the adsorption can prevent the Au-np against salt-induced aggregation.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2005 Conference Program Abstract

Gold Sponsors
Nanotech Gold Sponsors
Silver Sponsors
Nanotech Silver Sponsors
Gold Key Sponsors
Nanotech Gold Key Sponsors
Nanotech Ventures Sponsors
Nanotech Ventures Sponsors
Nanotech Sponsors
News Headlines
NSTI Online Community

© Nano Science and Technology Institute, all rights reserved.
Terms of use | Privacy policy | Contact