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The Large Scale Fabrication of Protein Nanoarrays Based on Nanosphere Lithography

Y. Cai and B.M. Ocko
Brookhaven National Laboratory, US

protein nanoarray nanosphere lithography, silane chemistry, AFM

Many emerging biological and medical applications involve the development of protein arrays at nanoscale.1 Dip-pen lithography2 nanografting and conducting tip AFM writing are capable of creating nano-protein patterns with 50-100 nm resolution over small surface regions. Large-scale patterning techniques, such as photolithography, micro-contact printing are used to pattern protein, but they lack the nanoscale resolution. We have combined nanosphere lithography and silane chemistry3 to chemically pattern macroscopic surface regions with nm scale features over cm2 sample sizes. Using self-assembled 300nm nanosphere monolayer as mask, we fabricated a hexagonal patterned COOH terminated holes on a PEG-terminated silane surface. Our results demonstrate that the protein lysozyme selectively has selectively adsorbed on the COOH terminated islands. The patterned protein islands covered the whole 1x1 cm2 wafer with an inter-island distance of 300nm. The protein islands exhibited a ring shape, with a ring diameter of 118nm and ring width of 50nm. This new method offers a fast and economical way to fabricate protein arrays over large areas with feature sizes comparable to scanning probe based techniques.

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