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Nano Science and Technology Institute 2005 NSTI Nanotechnology Conference & Trade Show
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Fast and Sensitive DNA Hybridization Through Micro- Fluidics

A.H. Marafie and M.J. Madou
University of California, Irvine, US

DNA hybridization, micro-fluidics

The research target for DNA hybridization through micro-fluidics is to achieve a fast and very sensitive hybridization reaction by investigating the effect of different pertinent parameters using computational and experimental methods. The mathematical model that we developed couples the mass transport of DNA from the bulk of the solution with the surface reaction kinetics for the DNA hybridization reaction in a micro fluidic channel. A two-dimensional (2-D) analysis of the classical mass transport equation under laminar flow conditions combined with an overall second order rate constant for the surface hybridization reaction elucidates the effect of changing the fluid flow rates, flow velocity, bulk concentration and channel heights on the DNA hybridization efficiency. The model enables the DNA researcher to optimize his DNA assays for speed and lower limit of detection.

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