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SERS Nanotags – a Highly Multiplexed Nanoparticulate Optical Detection Label for Biological Assays

S.G. Penn, R.G. Freeman, M. Sha, W. Doering and M.J. Natan
Nanoplex Technologies, US

nanoparticle, Raman, detection, optical, multiplexed

Nanoplex Technologies is applying nanotechnology in the development of the next generation of highly-multiplexed optical detection tags for life science research. SERS nanotags are glass-coated metal nanoparticulate tags that can be simultaneously quantified using surface enhanced Raman scattering (SERS). SERS nanotags offers numerous advantages over traditional optical detection tags, including the capacity for very high level multiplexing, the ability to be readily used in whole blood and tissue samples, unprecedented photostability, ease of bioconjugation, and uniform size. Therefore, we can address many of the multiplex measurement requirements required in cancer research, including multiplexed measurements of DNA, proteins, and intracellular measurement of cell signaling pathways, multiplexed whole blood diagnostics and point-of-care devices, and the potential for in vivo imaging. We will present data showing the utility of this nanotechnology for a variety of biological platforms including flow cytometry, cellular imaging, point of care diagnostics and microarrays – all important assay formats in the fight against cancer. This work was supported by the National Cancer Institute, through an SBIR grant (1R43CA111752-01).

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