Nano Science and Technology InstituteNano Science and Technology Institute
Nano Science and Technology Institute 2003 NSTI Nanotechnology Conference & Trade Show
Nanotech 2003
BioNano 2003
Index of Authors
2003 Sub Sections
Press Room
NSTI Events
Site Map
Nanotech Proceedings
Nanotechnology Proceedings
Supporting Organizations
Supporting Organizations
Event Contact
696 San Ramon Valley Blvd., Ste. 423
Danville, CA 94526
Ph: (925) 353-5004
Fx: (925) 886-8461

Solid State Nanopore as a Single DNA Molecule Detector

Jiali Li, Derek Stein, Cai Qun, Eric Brandin, Hui Wang, Albert Huang, Dan Branton and Jene Golovchenko
Department of Physics, Harvard University, US

Keywords: Solid-state nanopores devices, DNA molecule detection

We report on the use of nanometer scale diameter, solid state nanopores as single molecule detectors of double stranded DNA molecules. These solid-state nanopores are fabricated in thin membranes of silicon nitride, by ion beam sculpting 1. They produce discrete electronic signals when an electrically biased nanopore is exposed to DNA molecules in aqueous salt solutions. We demonstrate examples of such electronic signals for 3 KB and 10 KB double stranded DNA molecules which show that these molecules are individually translocating through the nanopore during the detection process. The translocating time for 10 KB double stranded DNA is about 3 times longer than the 3 KB, demonstrating that a solid state nanopore device can be used to detect the lengths of double stranded DNA molecules. Similarities and differences with signals obtained from single stranded DNA in a biological nanopores are discussed 2. 1. Jiali Li, Derek Stein, Ciaran McMullan, Daniel Branton, Michael J. Aziz, and Jene Golovchenko. Ion Beam Sculpting at nanometer length scales. Nature 412, 166-169 (2001). 2. Meller, A., L. Nivon, E. Brandin, Golovchenko, J. & Branton, D. Rapid nanopore discrimination between single polynucleotide molecules. Proc. Natl. Acad. Sci. USA 97, 1079-1084 (2000).

NSTI Nanotech 2003 Conference Technical Program Abstract

Featured Sponsors
Nanotech Sponsors
News Headlines
NSTI Online Community

© Nano Science and Technology Institute, all rights reserved.
Terms of use | Privacy policy | Contact