Authors: W. Zhu, L. Zhang
Affilation: The George Washington University, United States
Pages: 206 - 209
Keywords: electrospinng, electrospraying, nanoparticle, nanocomposite, neural tissue engineering
A novel tissue engineered scaffold was fabricated by electrospinning and electrospraying techniques, which possesses highly aligned poly-ε-caprolactone (PCL) microfibrous framework and adjustable neurotropic factor embedded poly (D, L-lactide-co-glycolide) (PLGA) nanospheres. Scanning electron microscope revealed the uniform highly aligned structure of the scaffolds, the PLGA nanospheres were loaded successfully as well. Contact angle test illustrated a more hydrophilic surface was created after PLGA nanospheres coated. Drug release study showed the PLGA nanospheres coated on the scaffold surface can keep a long term release behavior. The special nanostructured topography and embed neurotrophy have been verified to enhance PC12 cell proliferation, this can be ascribed to more cell adhesion mediating proteins absorption on the nanostructured scaffold surface. In addition, confocal microscope images illustrated the aligned nanocomposite scaffold directed the neurites extension along the fibers. This guidance shows great promise for the scaffold to support the new tissue grow to the target during neural regeneration.