Authors: K. Higashisaka, A. Kunieda, Y. Iwahara, K. Tanaka, S. Tsunoda, Y. Yoshioka, Y. Tsutsumi
Affilation: Graduate School of Pharmaceutical Sciences, Osaka University, Japan
Pages: 131 - 133
Keywords: nanomaterial, neutrophil
Nanomaterials (NM) with particle sizes below 100 nm display unique properties compared to conventional materials with a submicron size. On the other hand, the debate on safety of NM has expanded worldwide. Therefore, to fully utilize the potential benefits of NM, it is essential to obtain more information to ensure the safety of NM. In this regard, it has been focused on neutrophil being involved in some biological responses such as biodistribution and inflammatory responses after exposure to NM. However, their detail information about the involvement with neutrophil and NM-induced biological responses is still little. Here, we examined the changes of neutrophil proportion induced by silica nanoparticles and attempted to gather basic information about the association with silica nanoparticles-induced biological effects. BALB/c mice were treated intravenously of silica particles with diameters of 70 nm (nSP70) or saline. Our results suggested that nSP70 would induce neutrophil activation with an increase in proportion of neutrophil and that increasing neutrophil proportion induced by nSP70 depends on nSP70-induced G-CSF elevation. We are currently analyzing association between nSP70-induced neutropilia and some adverse biological effects or kinetics after nSP70 exposure. In the future, our study would contribute to develop the safety and efficacy NM.