Nano Science and Technology Institute
Nanotech 2014 Vol. 2
Nanotech 2014 Vol. 2
Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational & Photonics
 
Chapter 3: Biosensing, Diagnostics & Imaging
 

Nanostructured Memristor Sensor Mimics Acetylcholinesterase (ACHE) Active Sites in the Gorge for fM Detection of Acetylcholine

Authors:E.T. Chen, J. Thornton, C. Ngatchouand, S-H. Duh
Affilation:Advanced Biomimetic Sensors, Inc., US
Pages:169 - 172
Keywords:nanobiomimetic memristor sensing, acetylcholinesterase (ACHE) gorge, acetylcholine
Abstract:Many diseases including cancer, diabetes, brain injury, epilepsy, Parkinson’s, autism and Alzheimer’s involve the up regulation of acetylcholinesterase (ACHE) inappropriately signaling of cholinergic synapse for an excess production of the neurotransmitter acetylcholine (ACH). Challenges for providing a nonenzymatic detection device for ACH with rapid detection time and high sensitivity are paramount due to time consuming assays and protein interferences. We report two types of nanostructured memristor biomimetic ACHE sensors developed for detection of ACH in order to solve the challenges. Memristor sensor #1 was made for mimicking the normal active sites in the ACHE gorge, and memristor sensor #2 was made for mimicking the mutated gorge for direct detection of ACH without using antibody and no biomarkers. Multiple polymers were cross linked and self-assembled on gold chips forming a “vertical bridged-nanpore ACHE gorge for sensor #2; and formed a “flat-bridge/nanopore gorge for sensor #1. Frequency effects on the i-V curves were observed of hysteresis. Results obtained by an Amperometric (CA) method for quantitation of ACH revealed the sensor #1 has Detection of Limits of ACH with the DOL of 9.7x10-16 M/cm2 over 10-15 to 10-6 M. However, the sensor #2 was unable to detect ACH directly.
ISBN:978-1-4822-5827-1
Pages:570
Hardcopy:$209.95
 
Order:Mail/Fax Form
Up
© 2014 Nano Science and Technology Institute. All Rights Reserved.
Terms of Use | Privacy Policy | Contact Us | Site Map