Nano Science and Technology Institute
Nanotech 2014 Vol. 1
Nanotech 2014 Vol. 1
Nanotechnology 2014: Graphene, CNTs, Particles, Films & Composites
Chapter 4: Polymer Nanotechnology, Soft Matter & Colloids

Formation of complex nanoparticles from a spherical protein (BSA) and two polycations (PDL with two molecular weights) using coacervation method of the polyelectrolytes

Authors:L. Maldonado, R. Sadeghi, J. Kokini
Affilation:Purdue University, US
Pages:291 - 294
Keywords:complex nanoparticles, polyelectrolytes, electrostatic forces, coacervation method, DLS
Abstract:Complex nanoparticles (CNPs) were formed by mixing Bovine Serum Albumin (BSA) and Poly-D-Lysine with low (LMW-PDL) and high molecular weights (HMW-PDL). Since BSA and both PDL biopolymers in neutral and alkaline pHs are oppositely charged biopolymers, so they can easily interact by physically mixing of them. This interaction was established by measuring the zeta potential of BSA and both PDL biopolymers before and after mixing, and the interaction process was called complex coacervation process. The minimum effective diameter, in the range of 200 ± 1.40 nm and 215 ± 1.97 nm, was achieved with LMW-PDL dissolved in phosphate buffer saline with 0.1 M NaCl at pH 7 and a mass ratio of 2.0 (BSA: PDL). These particle sizes decreased at 101 ± 7.02 nm nm with the addition of 0.25% glutaraldehyde as cross-linking agent, but polydispersity index (PDI) increased to 0.47 ± 0.07. Nanoparticles (NPs) from HMW-PDL showed effective diameter in the range of 250 ± 32.8 nm and 300 ± 24.4 nm; with the addition of 0.25% glutaraldehyde, the particle size decreased to 200 ± 1.75 nm and 220 ± 3.05nm, but PDI increased to 0.319 ± 0.008
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