Nanotech 2013 Vol. 3
Nanotech 2013 Vol. 3
Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)

Bio Nano Materials Chapter 2

Investigations on Transmembrane Ion Channels Suspended over Porous Silicon Membranes

Authors: K.H. Tantawi, B. Berdiev, R. Cerro, J.D. Williams

Affilation: University of Alabama in Huntsville, United States

Pages: 198 - 201

Keywords: porous silicon, lipid bilayer, transmembrane ion channel, Impedance spectroscopy

Abstract:
This article presents a batch-fabricated porous silicon membrane tool used to investigate transmembrane protein functionality in complex biological systems. We address the physical and chemical requirements fabricate the thin porous membrane, attached a lipid bilayer, fuse an ion channel protein and measure the electrical response of the system. This was achieved through the use of electrochemical impedance analysis, atomic force microscopy, and specific optical spectroscopic methods. We demonstrate that integrating membrane protein functionality directly with silicon device technology can be used for energy conservation and electronic signaling both to and from a biological system. Finally, these studies will enable the test of multiple proteins working together within cell or organelle membranes.


ISBN: 978-1-4822-0586-2
Pages: 806
Hardcopy: $209.95