Nano Science and Technology Institute
Nanotech 2013 Vol. 3
Nanotech 2013 Vol. 3
Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Chapter 2: Bio Nano Materials

Aerosol-based fabrication of modified chitosans and their application for gene transfection

Authors:J.H. Byeon, J.T. Roberts
Affilation:Purdue University, US
Pages:179 - 182
Keywords:aerosol, modified chitosan, gene transfection
Abstract:Droplets containing modified chitosans were formed by collison atomization, dried to form solid particles, and collected and studied for potential use as nanocarriers (Figure 1). Modified chitosans consisted of a chitosan backbone and an additional component [with cholesterol, poly(L-lysine) (PLL), PEI, or poly(ethylene glycol) (PEG)]. Agarose gel retardation assays confirmed that modified chitosans could associate with plasmid DNA. Even though the cell viability of cholesterol-chitosan (Ch-Cs) showed a slight higher cytotoxicity (~90% in cell viability) than that for unmodified chitosan (Cs, ~95%), transfection [>7.5 × 105 in relative light units (RLU) mg-1] was more effective than it was for Cs (~7.6 × 104 RLU mg-1). The addition of PEI onto Cs (i.e. Cs/PEI) enhanced the transfection efficiency (~1.3 × 106 RLU mg-1) more than did the addition of PLL (i.e. Cs/PLL, ~9.3 × 105 RLU mg-1). However it resulted in higher cytotoxicity (~86% in cell viability for Cs/PEI vs ~94% for Cs/PLL). The cell viability (~92%) and transfection efficiency (~1.9 × 106 RLU mg-1) were complemented by further adding PEG on Cs/PEI (i.e. Cs/PEI-PEG). This work concludes that gene transfection of Cs can be significantly enhanced by adding cationic polymers during aerosol fabrication without wet chemical modification processes of Cs.
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