Authors: E.T. Chen, J. Thornton, C. Ngatchou, S-H. Duh, P.T. Kissinger
Affilation: Advanced Biomimetic Sensors, Inc., United States
Pages: 115 - 118
Keywords: nanobiomimetic sensing, direct electron transfer (DET) rate constants, correlation of direct electron transfer (DET) rate constants, anticancer candidate compounds
We report a new approach to study Direct Electron Transfer (DET) rate constants between an electrode surface and an active biomimetic receptor using four types of gold nanobiomimetic sensors. The goal is to find the correlation between DET and the ability to kill cancer cells using known anti-cancer compounds. The DET results were obtained under antibody-free, label-free and analyte-free conditions in cell culture solutions using a cyclic voltammetry (CV) method. Sensor 1 has 20 nm nanopores, Sensor 2 has 40 nm nanopores, Sensor 3 has nano islands and Sensor 4 has a vertical nano bridge with nanopores. The DET ks results are sensor 4>sensor 3>sensor1>sensor 2 with the highest result 287/s for sensor 4. Anti-cancer compounds 1-Methyl-2-piperidinemethanol (MPPDM) and Flavopiridol hydrochloride hydrate (FLAPHH) were used for evaluation of brain cancer cells of SNB-19 by a Chronoamperometric (CA) method. Results shown the MPPDM and FLPHH completely blocked cancer signaling with sensor 4 at 5 cancer cells/mL, while sensor 3 with lower DET was unable to detect at this level. Our preliminary results revealed that the higher DET values directly correlate to the higher cancer killing rates from the anti cancer compounds.