Authors: W. Choi, J.H. Lee
Affilation: Luminescent MD, LLC, United States
Pages: 729 - 732
Keywords: SNP, graphene oxide, carbon nanotubes, hybridization, chemiluminescence
Rapid single nucleotide polymorphism (SNP) with 1,1’-oxalylimidazole chemiluminescence (ODI-CL) detection was developed for the early diagnosis of human mad-cow disease. Mutated single strand DNA (ssDNA) sequence discovered from patients having human mad-cow disease rapidly hybridize with complementary probe conjugated with chemiluminescent dye. The hybridization between target ssDNA and complementary probe was dependent on four different variables such as pH, temperature, incubation time, and the type of nanoparticles (e.g., multi-walled carbon nanotubes, graphene oxide) capable of capturing mismatched ssDNAs and excess complementary probe. However, three different types of mismatched ssDNAs also slowly hybridize with complementary probe with the increase of incubation time. The problem was solved by using ODI-CL detection instead of fluorescence detection of conventional SNP because ODI-CL is about 100 times more sensitive than fluorescence. In other words, it was possible to reduce the interaction between mismatched ssDNAs and complementary probe with short incubation time (15 minutes) using highly sensitive ODI-CL detection. Based on the results, in conclusion, it is expected that SNP with ODI-CL can be applied as a cost-effective, rapid, and simple analytical method to diagnose and prognose various diseases.