Authors: H.K. Choi, L. Park, J. Kim and J.H. Lee
Affilation: Luminescent MD, LLC, United States
Pages: 713 - 716
Keywords: chemiluminescent aptasensor, magnetic graphene oxide, aptamer, tumor marker
Rapid and sensitive chemiluminescent aptasensor capable of rapidly sensing tumor markers in human serum with the combination of magnetic and non-magnetic graphene oxide. Human serum (150 µl) containing tumor markers (e.g., AFP and PSA) was mixed in PBS (pH 7.4, 10 µl) containing magnetic graphene oxide (MGO) and incubated for 30 minutes to remove various biomolecules acting as interferences of chemiluminescent aptasensor. Then, ssDNA aptamer-conjugated Cy 3 (100 µl), capable of capturing a specific tumor marker, in Tris-HCl buffer (pH 7.5) was added in the solution and incubated for 5 minutes. After the incubation, non-magnetic graphene oxide in Tris-HCl buffer (pH 7.5, 100 µl) was added in the solution to capture free ssDNA aptamers based on the principle of pi-pi stacking interaction. Finally, chemiluminescent aptasensor rapidly quantified tumor markers bound with ssDNA aptamer-conjugated Cy3 when 1,1’-oxalyldiimidazole (ODI) chemiluminescent reagents (e.g., ODI, H2O2). The correlation between highly accurate and sensitive chemiluminescent aptasensor and conventional enzyme immunoassay was good. Based on the research results, it is expected that chemiluminescent aptasensors can be developed to early diagnose and prognose various human diseases.